Notes from USGEB11

Thursday 27.01.2011

Seeing Cells in Action with photoactivatable fluorescent Proteins - Jennifer Lippincott-Schwartz

  • Photo-activated light microscopy → Super-resolution
  • Autophagy → self-destruction of cell
  • correlative imaging PALM/TEM → map molecules on Electron microscopy images
  • 3D imaging possible
    • wave from photon is propagated through two objectives
    • interference makes it possible to extract 3D position

Rolf Grütter - Imaging Brain Metabolism

  • Brain glucose level is linear to plasma glucose
  • Brain glucose content mapped using 1H NMR
  • Spectroscopy can be used to emasuera concentration of biochemical compounds
    • Concentration > 1 MM
    • water soluble
  • → quantitative tool

Corrado Corradi - felt and observed pain...

  • Analysis of activation patterns of brain regions of observed and experienced pain → localized in one single part of the brain

Beat Riederer - Imaging Brain Protein

Christophe Lamy - Imaging Sodium in Tissue

C. Cremer Nanoscopy of Nuclear Architecture

  • distribution of cells in Nucleus → Super-resolution
  • Merging of several images together

David Müller

  • AFM imaging

Friday 28.01.2011

Introduction

  • PET is quantitative, but had only a resolutin in the mm-range

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Windhorst - New PET Pharaceuticals in Oncology

  • Translational molecular imaging
  • Molecular labeling with different compounds
  • Makes personalized treatment possible, but needs testing for treatment with specific FDG-marker

Tony Gee - PET in the CNS

  • Since markers have short half life, repeated studies are possible
  • 11C has 20min half life
  • Enzymes can be used, even Cocaine, Heroin and Nicotine. Can even be done without the pharmacological effect, since the markers can be labeled (which destroys the effect?)
  • For antidepressants, a combination of Pindolol and SSRI was shown to improve onset of action. 70% of 5-HT1A - Receptor has to be occupied by Pindolol.

Surekha Pimple - Evaluation of Quinazoline as PET Tracers

  • Development and characterization of new tracers for PET imaging

Haibiao Gong - in vigo imaging of xenograft tumors

  • Near infrared imaging of tumors in vivo, follows by ex vivo imaging of the whole organ and tissue sectioning
  • Activation of Affibodies could be detected.

Jana Doehner - Role of Reelin in brain tissue

  • Plays a role in Alzheimer research
  • Expression of Reelin changes over aging
  • Similarities between human and mouse plaques

Fritjof Helmchen - Emerging techniques for in vivo imaging of Neural Circuit Dynamics

  • Neocortex has several layers of neurons until the white matter
  • Depth penetration into Neocortex around 800 um, into living tissue!
  • Single action potentials through imaging combined wit electrical recording
  • Two-photon imaging with 'swinging objective' to cover full ROI
  • Tradeoff between time resolution and size of Neuron population which can be imaged
  • Plasticity effect in the brain (due to trimming of whiskers)
  • Imaging in awake and behaving mice an be done with animals which are trained to tolerate head fixation