Monday morning - 3D X-ray Microscopy and Tomography

  • Order of magnitude increase of brilliance compared to SLS
  • MicroBunching of electrons > Self-amplifying spontaneous Emission (SASE, bettr word than FEL)
  • THz Pump source
  • detect Motion of Atoms on surfaces (ps imaging with Split-Pulse imaging)
  • avoid radiation damage, fast Imaging
  • semicondictors thermal Stress
  • tomograhpy with Full-Field x-Ray microscopes
  • cryo TXM, different Sample holder possibilities
  • Resolution down to 12 nm
  • higher Resolution, but less Penetration
  • quantitative Chemical information with high spatisl Resolution
  • Mapping of compounds in cells
  • Resolution Limit for 4D-imaging, since nanotomography is destructive
  • Resolution down to 250nm with FIB-grooves/holes in 3D. 100nm Features in 2D
  • complex multilayer microarchitecture of eggs
  • Pores/Channels in Egg-Shells
  • Scanning transmission xray microscopy
  • scattering Information from different Materials
  • scattering Information gives orientation of collagen in Bone
  • different information from the same dataset
  • elemental Mapping is possible
  • assessing revonstruction quality
  • colocation of elements and concentrations is possible, but scanning tomography is really slow
  • well suited for nanoscale investigation on small biological specimens
  • packed snow is far away from nice stars, no 3D architecture
  • snow is a sintered porous material with Ice and air phase
  • non-destructive measurement > time-lapse tomography > compression of snow over 5 days
  • grain boundary analysis in 3D
  • 3D watershed (Soille 1999, Godehardt 2000 (?))
  • Skeletonization (Ioanjidis 2000, Liang 2000)
  • 3D-map of chemical composition, again slow
  • method without synchrotron > 50 um pinhole in front of fluorescence detector (XRF CCD > energy detector)
  • detection of elements from ~3-30 keV

Monday afternoon - 3D CLSM and Light Microscopy

  • technical aspects of confocal microscopy
  • deconvolution
  • multiple-photon laser
  • how do branched epithelial networks form during development?
  • cell rearrangement made visible with confocal microscopy
  • exposure to particle aerosols > uptake mechanism
  • engineered nanoparticles can be images by advanced microscopic methods
  • confocal tomography
  • ixcom20.fzk.de
  • confocal laser scanning microscope combined with focused ion beam SEM
  • milling and imaging > 3D image stack with EM-resolution
  • FIB milling to obtain 3D electron microscopy tomographic datadet