====== Notes from USGEB11 ======
[[http://www.usgeb2011.uzh.ch/|USGEB Meeting 2011, Zurich «Imaging Life»]]
====== Thursday 27.01.2011 ======
===== Plenary Lecture I =====
Seeing Cells in Action with photoactivatable fluorescent Proteins - Jennifer Lippincott-Schwartz
* Photo-activated light microscopy -> Super-resolution
* Autophagy -> self-destruction of cell
* correlative imaging [[https://secure.wikimedia.org/wikipedia/en/wiki/Microscopy#PALM.2C_STORM|PALM]]/TEM -> map molecules on Electron microscopy images
* 3D imaging possible
* wave from photon is propagated through two objectives
* interference makes it possible to extract 3D position
===== Brain Imaging - Symposium D =====
==== Rolf Grütter - Imaging Brain Metabolism ====
* Brain glucose level is linear to plasma glucose
* Brain glucose content mapped using 1H [[wp>NMR]]
* Spectroscopy can be used to emasuera concentration of biochemical compounds
* Concentration > 1 MM
* water soluble
* -> quantitative tool
==== Corrado Corradi - felt and observed pain... ====
* Analysis of activation patterns of brain regions of observed and experienced pain -> localized in one single part of the brain
==== Beat Riederer - Imaging Brain Protein ====
==== Christophe Lamy - Imaging Sodium in Tissue ====
===== Exploring Biological Matter by Different Imaging Modalities - Symposium H =====
==== C. Cremer Nanoscopy of Nuclear Architecture ====
* distribution of cells in Nucleus -> Super-resolution
* Merging of several images together
==== David Müller ====
* AFM imaging
====== Friday 28.01.2011 ======
===== Imaging life using PET - Symposium J =====
==== Introduction ====
* PET is quantitative, but had only a resolutin in the mm-range
==== Windhorst - New PET Pharaceuticals in Oncology ====
* Translational molecular imaging
* Molecular labeling with different compounds
* Makes personalized treatment possible, but needs testing for treatment with specific FDG-marker
==== Tony Gee - PET in the CNS ====
* Since markers have short half life, repeated studies are possible
* 11C has 20min half life
* Enzymes can be used, even Cocaine, Heroin and Nicotine. Can even be done without the pharmacological effect, since the markers can be labeled (which destroys the effect?)
* For antidepressants, a combination of Pindolol and SSRI was shown to improve onset of action. 70% of 5-HT1A - Receptor has to be occupied by Pindolol.
==== Surekha Pimple - Evaluation of Quinazoline as PET Tracers ====
* Development and characterization of new tracers for PET imaging
==== Haibiao Gong - in vigo imaging of xenograft tumors ====
* Near infrared imaging of tumors in vivo, follows by ex vivo imaging of the whole organ and tissue sectioning
* Activation of [[http://en.wikipedia.org/wiki/Affibody_molecule|Affibodies]] could be detected.
==== Jana Doehner - Role of Reelin in brain tissue ====
* Plays a role in Alzheimer research
* Expression of Reelin changes over aging
* Similarities between human and mouse plaques
===== Plenary Lecture =====
==== Fritjof Helmchen - Emerging techniques for in vivo imaging of Neural Circuit Dynamics ====
* Neocortex has several layers of neurons until the white matter
* Depth penetration into Neocortex around 800 um, into living tissue!
* Single action potentials through imaging combined wit electrical recording
* Two-photon imaging with 'swinging objective' to cover full ROI
* Tradeoff between time resolution and size of Neuron population which can be imaged
* Plasticity effect in the brain (due to trimming of whiskers)
* Imaging in awake and behaving mice an be done with animals which are trained to tolerate head fixation